Integrated Biomimetic 2D-LC and Permeapad^® Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds.

ABSTRACT: A comprehensive two-dimensional liquid chromatography platform (LC × LC) was developed and validated for dermal permeability studies. In this implementation, the two separation dimensions were applied to mimic the layered structure of human skin: a ceramide-like stationary phase in the first dimension (¹D) to simulate the lipid-rich epidermis, and an immobilized artificial membrane (IAM) phase in the second (²D) to emulate the dermis. Experimental conditions were optimised to reflect the microenvironment of the in vivo skin. For validation purposes, 43 pharmaceutical and cosmetic compounds whose transdermal permeability coefficients (log K_p) were known from the scientific literature were selected as model solutes. A good degree of separation was achieved ac

SUBMITTER: Neri I 

PROVIDER: S-EPMC12843750 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature