CDK1-driven phosphorylation networks promote glioblastoma progression via MAP1B-mediated microtubule destabilization.

ABSTRACT:

Background

Glioblastoma (GBM) is the most aggressive and prevalent malignant brain tumor in adults, with poor prognosis despite current therapies. Cyclin-dependent kinase 1 (CDK1), a master regulator of cell cycle progression, has been implicated in oncogenesis, but its downstream phosphorylation network in GBM remains incompletely defined.

Methods

CDK1 expression was examined in clinical GBM tissues and cell lines. Functional studies were performed in U251 cells using CDK1-specific shRNAs. Label-free phosphoproteomic profiling and bioinformatics analyses were conducted to map CDK1-regulated signaling pathways and substrates. Prognostic associations were evaluated using Clinical Proteomic Tumor Analysis Consortium (CPTAC) datasets, and functional assays were used to validate

SUBMITTER: Li JT 

PROVIDER: S-EPMC12846947 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature