Proteomics and acetyl-proteomics reveal the antibacterial mechanism of berberine sulfate against methicillin-resistant Staphylococcus aureus.

ABSTRACT:

Introduction

The rising prevalence of methicillin-resistant Staphylococcus aureus (MRSA) underscores an urgent need for novel antibacterial strategies. Although the natural antibacterial agent berberine sulfate exhibits inhibitory effects against MRSA, its precise molecular targets and mechanisms of action remain unclear.

Methods

To elucidate its mechanism, this study employed quantitative proteomics to analyze protein expression changes in MRSA before and after drug treatment. Acetylomics was further applied to investigate the impact on post-translational modifications. Key findings were functionally validated using site-directed mutagenesis and electrophoretic mobility shift assays (EMSA).

Results

Quantitative proteomics identified 255 differentially expresse

SUBMITTER: Hu Z 

PROVIDER: S-EPMC12847299 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature