DUSP22 dephosphorylates LGALS1 to enhance T cell-driven antitumor immunity.

ABSTRACT:

Background

Insufficient infiltration of CD8⁺ T cells in the tumor microenvironment (TME) critically restricts antitumor immunity and cancer immunotherapy efficacy. The purpose of this study was to identify novel tumor cell-intrinsic regulators of T-cell infiltration and to elucidate their mechanisms of action.

Methods

We performed a genome-wide Sleeping Beauty transposon mutagenesis screen in murine breast cancer models. Protein-protein interactions were identified by mass spectrometry and validated by co-immunoprecipitation. Gene and protein expression levels were assessed by reverse transcription and quantitative PCR and western blotting. T-cell infiltration and function were evaluated using flow cytometry, immunohistochemistry (IHC), multiplex IHC, and by analy

SUBMITTER: Wang L 

PROVIDER: S-EPMC12853559 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature