The RNA-binding protein RNP1A is essential and interacts with contractility kit proteins to facilitate cell mechanics.

ABSTRACT: Cell shape regulation is important for many biological processes. Some cell shape-regulating proteins harbor mechanoresponsive properties that enable them to sense and respond to mechanical cues. In Dictyostelium discoideum, mechanoresponsive network proteins formed by proteins such as myosin II, cortexillin I and IQGAP1 assemble in the cytoplasm into macromolecular complexes, which we term contractility kits (CKs). In our previous studies, we identified the RNA-binding protein RNP1A as a genetic interactor with the cytoskeletal machinery of the cell and as a biochemical interactor of cortexillin I, using in vivo fluorescence cross-correlation spectroscopy. In this study, we show that Dictyostelium rnp1A knockdown cells have reduced cell proliferation, reduced adhesion, defective cytokines

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12863306 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature