Uridine diphosphate drives myeloid differentiation and functional reprogramming through dynamic transcriptional network.

ABSTRACT:

Background

Extracellular nucleotides regulate immune responses through purinergic signaling. Uridine diphosphate (UDP), a pyrimidine-derived metabolite, has been shown to accumulate in the tumor microenvironment and modulate T cell activation. However, its effects on human myeloid cells remain poorly understood. Since monocytes represent key precursors for macrophages and dendritic cells, we investigated whether UDP could influence their proliferative and differentiation potential within peripheral blood mononuclear cells (PBMCs).

Methods

Freshly isolated PBMCs were stimulated with UDP, and CD14⁺ cell proliferation was analyzed using CFSE staining and flow cytometry. The impact of UDP on dendritic differentiation was evaluated in PBMC cultures and in purified CD14

SUBMITTER: Giordano C 

PROVIDER: S-EPMC12901406 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature