Characterization and production of a <i>Bacillus mycoides</i> Bioflocculant for sustainable effluent treatment.

ABSTRACT: Industrial effluent treatment relies on chemical coagulants, which incur higher costs and generate toxic sludge. This study isolated and identified bioflocculant-producing Bacillus mycoides (S39) strain and enhanced yield using one-factor-at-a-time optimization. Characterization studies of purified bioflocculant reveal a crystalline polysaccharide bearing amine, hydroxyl, and carboxylate groups that drive adsorption and polymer bridging. The bioflocculant exhibited flocculation activities of 95.56 % for textile effluent and 92.84 % for steel wastewater with Ca²⁺ activation. Bioflocculant remained stable across pH and temperature. Kinetics followed a pseudo-first-order model, yielding rate constants, an optimized dosage, and assistance with sizing. Hemolysis (<5 %) and via

SUBMITTER: Harinisri K 

PROVIDER: S-EPMC12907645 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature