Multistep metabolic engineered <i>Klebsiella oxytoca</i> for efficient l-leucine production.

ABSTRACT: l-Leucine is widely applied in food, feed and medical industries. In this work, an efficient l-leucine producing strain Klebsiella oxytoca LKO-14 was constructed based on an l-valine producer K. oxytoca VKO-9. The exogenous l-leucine biosynthesis pathway was introduced to achieve l-leucine accumulation and decrease l-valine production. Modifying l-leucine transport system and optimizing copy numbers of genes including CgleuA ^M encoding l-leucine insensitive isopropylmalate synthase, EcleuCD encoding isopropylmalate isomerase, EcleuB encoding isopropylmalate dehydrogenase, NopheDH encoding phenylalanine dehydrogenase, were conducted to increase l-leucine production. The expression of budB encoding α-acetolactate synth

SUBMITTER: Sun W 

PROVIDER: S-EPMC12907686 | biostudies-literature | 2026 Sep

REPOSITORIES: biostudies-literature