Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.

ABSTRACT:

Background

As the ancestor of CRISPR-Cas12 nucleases, TnpB represents the most compact gene editing tool currently available. Recent studies have identified multiple TnpB systems with gene editing activity in mammalian cells, and the potential of TnpB in treating diseases has been demonstrated in animal models. However, the editing characteristics of various TnpB systems, comparable to CRISPR tools, require more extensive investigation.

Results

Using a standardized evaluation framework, we conduct a thorough analysis of the editing properties of four TnpB variants alongside representative Cas12 and Cas9 tools applications. Overall, TnpBs exhibit intermediate editing activity and safety profiles among all tested systems, with ISYmu1 TnpB demonstrating a good performance in bo

SUBMITTER: Xin C 

PROVIDER: S-EPMC12908284 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature