Novel GABAAR antagonists target networked gene hubs at the leading edge in high-grade gliomas.

ABSTRACT:

Background

Ion channel activity underlying biological processes that drive high-grade gliomas (HGG) is largely unknown. We aimed to determine the networking of ion channel genes and validate their expression within HGG patient tumors, to identify ion channel-targeting drugs that would inhibit tumor-promoting processes.

Methods

We used weighted gene co-expression network analysis (WGCNA) of RNAseq data to identify ion channel gene hubs in diffuse midline glioma (DMG) and glioblastoma. Using scRNA-seq, spatial transcriptomics, and immunohistochemistry, we characterized the expression of identified hubs within patient tumors, validating their role by testing the efficacy of ion channel inhibitors alone or in combination with radiation and temozolomide on the growth and invasion

SUBMITTER: Shard C 

PROVIDER: S-EPMC12908485 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature