Mechanism and evolutionary divergence of a novel oxidized polyvinyl alcohol hydrolase in Stenotrophomonas rhizophila QL-P4.

ABSTRACT: Polyvinyl alcohol (PVA) is a widely used industrial polymer, and its persistence in the environment poses significant challenges, making the study of its biodegradation both a major production concern and an important scientific issue. This study aimed to elucidate the enzymatic mechanisms of PVA biodegradation by identifying and characterizing a novel oxidized polyvinyl alcohol hydrolase (OPH) from Stenotrophomonas rhizophila QL-P4, and to investigate its evolutionary divergence across microbial species. Through genomic analysis, we identified a gene, BAY15_0160 (996 bp), encoding a putative OPH. Its essential role in PVA degradation was confirmed via gene deletion, overexpression, and functional complementation assays. Site-directed mutagenesis demonstrated that the signal peptide and ac

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC12909831 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature