Role of CNNM4 in the progression of cholangiocarcinoma: implications for ferroptosis and therapeutic potential.

ABSTRACT:

Background and objective

Cholangiocarcinoma (CCA) is a heterogeneous neoplasm of the biliary epithelium that easily infiltrates, metastasises and recurs. Magnesium disbalance is a hallmark of CCA, with the magnesium transporter cyclin M4 (CNNM4) being a key driver of various hepatic diseases. This study aims to unravel the role of CNNM4 in the initiation and progression of CCA.

Design

CNNM4 protein and gene expression were assessed in vitro, in vivo and in patients with CCA. Silencing of CNNM4 was effectively achieved by using small interfering RNA (siRNA) or short hairpin RNA in CCA cell lines and GalNAc-conjugated siRNA in a transposon-based CCA mice model. The impact of CNNM4 on tumour cell proliferation, migration and invasion to the lungs was evaluated using the

SUBMITTER: Mercado-Gomez M 

PROVIDER: S-EPMC12911659 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature