Camsap2a regulates actomyosin flow and Rab5ab-mediated macropinocytosis in the yolk cell during zebrafish epiboly.

ABSTRACT: In zebrafish, epiboly is a major morphogenic event during gastrulation, characterized by the thinning and spreading of the embryonic blastoderm to internalize the underlying extra-embryonic yolk cell. This movement is driven by the yolk cell, which generates motile force through actomyosin flow that engages a circumferential contractile band, pulling the attached blastoderm vegetally. Localized macropinocytosis of the yolk cell, another actin-driven process, also contributes to epiboly progression by removing yolk membrane ahead of the advancing blastoderm. The molecular mechanisms coordinating these processes are elusive. Here, we identified Camsap2a, a non-centrosomal, microtubule-stabilizing protein, as a regulator of actin-dependent processes in the yolk cell during epiboly. Epiboly is

SUBMITTER: Wan H 

PROVIDER: S-EPMC12912265 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature