An essential gene screening identifies yeast Mot1 as a suppressor of R-loops and genome instability.

ABSTRACT: Transcription is essential for cellular function, but it can also lead to genetic instability, particularly through the formation of secondary structures such as R-loops, which consist of an RNA-DNA hybrid and a displaced DNA strand. Unscheduled R-loop accumulation is a major source of DNA damage and has been associated with several human diseases, including cancer. While multiple factors involved in RNA biogenesis, export, and chromatin remodeling play a role in preventing R-loop accumulation, the function of essential proteins in R-loop metabolism remains unexplored. Here, we performed a genetic screening in Saccharomyces cerevisiae using over 1200 temperature-sensitive mutants to identify novel proteins involved in the prevention of R-loop-associated genomic instability. Our results rev

SUBMITTER: Soler-Oliva ME 

PROVIDER: S-EPMC12912698 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature