Strong Basal/Tonic TCR Signals Are Associated with Negative Regulation of Naive CD4⁺ T Cells.

ABSTRACT: T cells experience varying intensities of tonic or basal TCR signaling in response to self-peptides presented by MHC (self-pMHC) in vivo. We analyzed four subpopulations of mouse naive CD4⁺ cells that express different levels of Nur77-GFP and Ly6C, surrogate markers that positively and inversely correlate with the strength of tonic TCR signaling, respectively. Adoptive transfer studies suggest that relatively weak or strong Nur77-GFP intensity in thymocytes tends to be maintained in mature T cells. Two-dimensional affinity measurements were lowest for Nur77-GFP^loLy6C⁺ cells and highest for Nur77-GFP^hiLy6C^- cells, highlighting a positive correlation between apparent TCR affinity and tonic TCR signal strength. Despite experiencing the st

SUBMITTER: Zinzow-Kramer WM 

PROVIDER: S-EPMC12917752 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature