T-Cell-Derived Exosomes From Multi Core Granules Exhibit Superior Caspase-3-Mediated Tumor-Suppressive Activity Compared to Those From Multivesicular Bodies.

ABSTRACT: Small extracellular vesicles (sEVs) derived from cytotoxic T lymphocytes (CTLs) are emerging as potential mediators of antitumor immunity; however, their subcellular origins and functional properties remain incompletely defined. In this study, we investigated the intracellular routes and cytotoxic potential of CTL-derived exosomes. Using correlative light and electron microscopy, we discovered that CTL-derived exosomes originate from both classical multivesicular bodies (MVBs) and the recently identified multi core granules (MCGs). Through total internal reflection fluorescence microscopy, we demonstrated that, in contrast to MVB-derived exosomes, MCG-derived exosomes are released at the immunological synapse in a stimulus-dependent manner. To enable functional characterization, we develop

SUBMITTER: Alawar N 

PROVIDER: S-EPMC12919372 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature