Interleukin-7 induces EMT to promote tumor growth and metastasis in NSCLC via Notch1/TGF-β pathway.

ABSTRACT: Interleukin 7 (IL‑7) regulates lymphangiogenesis and proliferation, inhibits apoptosis and autophagy in non‑small cell lung cancer (NSCLC). However, the role and detailed molecular mechanism of IL‑7 involved in NSCLC epithelial-to-mesenchymal transition (EMT) remain unknown. In this study, 119 cases of NSCLC tissue specimens were used to determine the expression levels and prognostic values of IL-7, IL-7R, E-cadherin and Vimentin. The CCK8, wound healing and transwell migration and invasion assays were employed to detect the NSCLC cell proliferation, migration and invasion, respectively. A subcutaneous tumor model and tail vein tumor injection in C57BL/6J mice were used to assess the role of IL-7 in vivo. Western blot, qRT-PCR and phalloidin staining assays were performed to investigate th

SUBMITTER: Shao Y 

PROVIDER: S-EPMC12923675 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature