Nitric oxide mediates ET-1-induced-inhibition of NPPB-sensitive Cl^- currents in the early distal convoluted tubule of the mouse kidney.

ABSTRACT: Endothelin-1 (ET-1) from renal-tubule-epithelial-cells inhibits NaCl reabsorption via ET_B receptor in an autocrine manner, and inhibition of ET_B receptors leads to salt-sensitive-hypertension. In the distal convoluted tubule (DCT), NaCl enters the cell via NaCl-cotransporter (NCC) and Cl^- exits the cell in part by ClC-K2 channels, which play a role in regulating With-No-lysine kinase 4 (WNK4). The study aims to explore whether ET-1-induced inhibition of NaCl absorption is also achieved by inhibiting the basolateral Cl^- channels in the DCT. Patch-clamp and immunoblotting assessed ET-1 effects on DCT Cl^- channels and NCC. Immunofluorescence images detected ETB-receptor expression in parvalbumin-positive DCT. Application of ET-1 decreased

SUBMITTER: Hu L 

PROVIDER: S-EPMC12925561 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature