A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell.

ABSTRACT: Regulation of translation is critical for the accurate expression of a broad variety of genes that function in cell cycle progression and cell differentiation, as well as in the adaptation to cellular stress. The aetiologies of a number of human diseases, including cancer, have been linked to mutations in genes that control mRNA translation, or in cis-regulatory mRNA-sequences. Therefore, research on translational control and its therapeutic appliance has become most important. However, to date only a limited number of therapeutic drugs are known to affect translational control. Here we describe a novel, straightforward approach for the detection of cellular translational activity. We developed a Translational Control Reporter System (TCRS), which utilizes the cis-regulatory upstream open

SUBMITTER: Wiesenthal V 

PROVIDER: S-EPMC1363784 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature