Identification of a copper-inducible promoter for use in ectopic expression in the fungal pathogen Histoplasma capsulatum.

ABSTRACT: Despite the existence of a number of genetic tools to study the fungal pathogen Histoplasma capsulatum, strategies for conditional gene expression have not been developed. We used microarray analysis to identify genes that are transcriptionally induced or repressed by the addition of copper sulfate (CuSO(4)) to H. capsulatum yeast cultures. One of these genes, CRP1, encodes a putative copper efflux pump that is significantly induced in the presence of CuSO(4). The upstream regulatory region of CRP1 was sufficient to drive copper-regulated expression of two reporter genes, lacZ and the gene encoding green fluorescent protein. Microarray experiments were performed to determine a copper concentration that triggers accumulation of the CRP1 transcript without significant perturbation of global

SUBMITTER: Gebhart D 

PROVIDER: S-EPMC1489277 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature