Rapid identification of Escherichia coli pathotypes by virulence gene detection with DNA microarrays.

ABSTRACT: One approach to the accurate determination of the pathogenic potential (pathotype) of isolated Escherichia coli strains would be through a complete assessment of each strain for the presence of all known E. coli virulence factors. To accomplish this, an E. coli virulence factor DNA microarray composed of 105 DNA PCR amplicons printed on glass slides and arranged in eight subarrays corresponding to different E. coli pathotypes was developed. Fluorescently labeled genomic DNAs from E. coli strains representing known pathotypes were initially hybridized to the virulence gene microarrays for both chip optimization and validation. Hybridization pattern analysis with clinical isolates permitted a rapid assessment of their virulence attributes and determination of the pathogenic group to which th

SUBMITTER: Bekal S 

PROVIDER: S-EPMC154688 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature