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Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway.

ABSTRACT: The Notch signaling pathway regulates the commitment and early development of T lymphocytes. We studied Notch-mediated induction of the pre-T cell receptor alpha (pTa) gene, a T-cell-specific transcriptional target of Notch. The pTa enhancer was activated by Notch signaling and contained binding sites for its nuclear effector, CSL. Mutation of the CSL-binding sites abolished enhancer induction by Notch and delayed the up-regulation of pTa transgene expression during T cell lineage commitment. These results show a direct mechanism of stage- and tissue-specific gene induction by the mammalian Notch/CSL signaling pathway.

SUBMITTER: Reizis B

PROVIDER: S-EPMC155334 | biostudies-literature | 2002 Feb

REPOSITORIES: biostudies-literature

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Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway.

Reizis Boris B Leder Philip P

Genes & development 20020201 3

The Notch signaling pathway regulates the commitment and early development of T lymphocytes. We studied Notch-mediated induction of the pre-T cell receptor alpha (pTa) gene, a T-cell-specific transcriptional target of Notch. The pTa enhancer was activated by Notch signaling and contained binding sites for its nuclear effector, CSL. Mutation of the CSL-binding sites abolished enhancer induction by Notch and delayed the up-regulation of pTa transgene expression during T cell lineage commitment. Th ...[more]

PMID: 11825871

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