Reverse engineering gene networks: integrating genetic perturbations with dynamical modeling.

ABSTRACT: While the fundamental building blocks of biology are being tabulated by the various genome projects, microarray technology is setting the stage for the task of deducing the connectivity of large-scale gene networks. We show how the perturbation of carefully chosen genes in a microarray experiment can be used in conjunction with a reverse engineering algorithm to reveal the architecture of an underlying gene regulatory network. Our iterative scheme identifies the network topology by analyzing the steady-state changes in gene expression resulting from the systematic perturbation of a particular node in the network. We highlight the validity of our reverse engineering approach through the successful deduction of the topology of a linear in numero gene network and a recently reported model for

SUBMITTER: Tegner J 

PROVIDER: S-EPMC156306 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature