Identification of two-component regulatory systems in Bifidobacterium infantis by functional complementation and degenerate PCR approaches.

ABSTRACT: Two-component signal transduction systems (2CSs) are widely used by bacteria to sense and adapt to changing environmental conditions. With two separate approaches, three different 2CSs were identified on the chromosome of the probiotic bacterium Bifidobacterium infantis UCC 35624. One locus was identified by means of functional complementation of an Escherichia coli mutant. Another two were identified by PCR with degenerate primers corresponding to conserved regions of one protein component of the 2CS. The complete coding regions for each gene cluster were obtained, which showed that each 2CS-encoding locus specified a histidine protein kinase and an assumed cognate response regulator. Transcriptional analysis of the 2CSs by Northern blotting and primer extension identified a number of put

SUBMITTER: MacConaill LE 

PROVIDER: S-EPMC165215 | biostudies-literature | 2003 Jul

REPOSITORIES: biostudies-literature