Low level microsatellite instability may be associated with reduced cancer specific survival in sporadic stage C colorectal carcinoma.

ABSTRACT:

Background

Colorectal cancers (CRCs) may be categorised according to the degree of microsatellite instability (MSI) exhibited, as MSI-high (MSI-H), MSI-low (MSI-L), or microsatellite stable (MSS). MSI-H status confers a survival advantage to patients with sporadic CRC.

Aims

To determine if low levels of MSI are related to the clinicopathological features and prognosis of sporadic stage C CRC.

Patients

A total of 255 patients who underwent resection for sporadic stage C CRC were studied. No patient received chemotherapy. Minimum follow up was five years.

Methods

DNA extracted from archival malignant and non-malignant tissue was amplified by polymerase chain reaction using a panel of 11 microsatellites. MSI-H was defined as instability at > or =40% of markers, MS

SUBMITTER: Wright CM 

PROVIDER: S-EPMC1774365 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature