Dataset Information

Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart.

ABSTRACT:

Background

In order to further distinguish unique from general functions of connexin43, we have generated mice in which the coding region of connexin43 was replaced by that of connexin26.

Results

Heterozygous mothers showed impaired mammary gland development responsible for decreased lactation and early postnatal death of the pups which could be partially rescued by wild type foster mothers. Only about 17% of the homozygous connexin43 knock-in connexin26 mice instead of 25% expected according to Mendelian inheritance, were born and only 6% survived to day 21 post partum and longer. Neonatal and adult connexin43 knock-in connexin26 mice exhibited slowed ventricular conduction in their hearts, i.e. similar but delayed electrophysiological abnormalities as connexin43 deficient mice. Furthermore, connexin43 knock-in connexin26 male and female mice were infertile and exhibited hypotrophic gonads. In testes, tubuli seminiferi were developed and spermatogonia as well as some primary spermatocytes were present, but further differentiated stages of spermatogenesis were absent. Ovaries of female connexin43 knock-in connexin26 mice revealed only few follicles and the maturation of follicles was completely impaired.

Conclusion

The impaired gametogenesis of homozygous males and females can explain their infertility.

SUBMITTER: Winterhager E

PROVIDER: S-EPMC1852306 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Publications

Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart.

Winterhager Elke E Pielensticker Nicole N Freyer Jennifer J Ghanem Alexander A Schrickel Jan W JW Kim Jung-Sun JS Behr Rüdiger R Grümmer Ruth R Maass Karen K Urschel Stephanie S Lewalter Thorsten T Tiemann Klaus K Simoni Manuela M Willecke Klaus K

BMC developmental biology 20070404

<h4>Background</h4>In order to further distinguish unique from general functions of connexin43, we have generated mice in which the coding region of connexin43 was replaced by that of connexin26.<h4>Results</h4>Heterozygous mothers showed impaired mammary gland development responsible for decreased lactation and early postnatal death of the pups which could be partially rescued by wild type foster mothers. Only about 17% of the homozygous connexin43 knock-in connexin26 mice instead of 25% expect ...[more]

PMID: 17408477

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Dataset Information

Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart.

Background

Results

Conclusion

Publications

Replacement of connexin43 by connexin26 in transgenic mice leads to dysfunctional reproductive organs and slowed ventricular conduction in the heart.

Similar Datasets

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