Rapid determination of macrolide and lincosamide resistance in group B streptococcus isolated from vaginal-rectal swabs.

ABSTRACT:

Objective

Our objective was to assess the ability of real-time PCR to predict in vitro resistance in isolates of group B streptococcus (GBS).

Methods

The first real-time PCR assays for the genes known to confer resistance to erythromycin and clindamycin in GBS were developed. Three hundred and forty clinical GBS isolates were assessed with these assays and compared with conventional disk diffusion.

Results

The presence of an erythromycin ribosome methylation gene (ermB or ermTR variant A) predicted in vitro constitutive or inducible resistance to clindamycin with a sensitivity of 93% (95% CI 86%-97%), specificity of 90% (95% CI 85%-93%), positive predictive value of 76% (95% CI 67%-84%), and negative predictive value of 97% (95% CI 94%-99%).

Conclusion

This rap

SUBMITTER: Dela Cruz WP 

PROVIDER: S-EPMC1939917 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature