A critical role of serum response factor in myofibroblast differentiation during experimental oesophageal ulcer healing in rats.

ABSTRACT:

Background

Myofibroblast differentiation is a key event during wound healing and is triggered primarily by transforming growth factor beta (TGFbeta). Serum response factor (SRF) is a TGFbeta-inducible transcription factor that is important for wound healing. Injection of SRF expression plasmid into rat gastric ulcers significantly accelerated restoration of epithelium and smooth muscle structures.

Aim

To determine the role of SRF in oesophageal ulcer healing, especially in myofibroblast differentiation.

Subjects

Rats (in vivo), oesophageal epithelial cells (Het1A) and fibroblasts (Rat1-R12) (in vitro) were used.

Methods

Oesophageal ulcers were induced in rats with acetic acid and subsequently treated by local injection of plasmids expressing either SRF or SRF a

SUBMITTER: Chai J 

PROVIDER: S-EPMC1942159 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature