Evidence for a minimal eukaryotic phosphoproteome?

ABSTRACT:

Background

Reversible phosphorylation catalysed by kinases is probably the most important regulatory mechanism in eukaryotes.

Methodology/principal findings

We studied the in vitro phosphorylation of peptide arrays exhibiting the majority of PhosphoBase-deposited protein sequences, by factors in cell lysates from representatives of various branches of the eukaryotic species. We derived a set of substrates from the PhosphoBase whose phosphorylation by cellular extracts is common to the divergent members of different kingdoms and thus may be considered a minimal eukaryotic phosphoproteome. The protein kinases (or kinome) responsible for phosphorylation of these substrates are involved in a variety of processes such as transcription, translation, and cytoskeletal reorganisation

SUBMITTER: Diks SH 

PROVIDER: S-EPMC1945084 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature