Control of transposase activity within a transpososome by the configuration of the flanking DNA segment of the transposon.

ABSTRACT: The multiple steps of DNA transposition take place within a large complex called the transpososome, in which a pair of transposon DNA ends are synapsed by a multimer of the transposase protein. The final step, a DNA strand transfer reaction that joins the transposon ends to the target DNA strands, entails no net change in the number of high-energy chemical bonds. Physiology demands that, despite remaining stably associated with the transpososome, the strand transfer products undergo neither the reverse reaction nor any further cleavage reactions. Accordingly, when the Mu or Tn10 strand transfer complex was produced in vitro through transposase-catalyzed reaction steps, reverse reactions were undetectable. In contrast, when the Mu or Tn10 strand transfer complexes were assembled from DNA al

SUBMITTER: Mizuuchi M 

PROVIDER: S-EPMC1964541 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature