Osteopontin regulates ubiquitin-dependent degradation of Stat1 in murine mammary epithelial tumor cells.

ABSTRACT:

Background

Osteopontin (OPN) is a secreted glycoprotein that mediates cell-matrix interactions and cellular signaling by binding with integrin (primarily alpha(v)beta(3)) and CD44 receptors. OPN regulates cell adhesion, chemotaxis, macrophage-directed IL-10 suppression, stress-dependent angiogenesis, apoptosis prevention, and anchorage-independent growth of tumor cells. However, the molecular mechanisms that define the role of OPN in tumor progression and metastasis are incompletely understood.

Methods

In this study, we use a system of 4T1 and 4T07 murine mammary epithelial tumor cell lines that are divergent in both metastatic phenotype and OPN expression. 4T1 expresses OPN and hematogeneously metastasizes, whereas 4T07 does not express OPN and is highly tumorigenic but fai

SUBMITTER: Gao C 

PROVIDER: S-EPMC1993854 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature