Application of comparative genomics in the identification and analysis of novel families of membrane-associated receptors in bacteria.

ABSTRACT:

Background

A great diversity of multi-pass membrane receptors, typically with 7 transmembrane (TM) helices, is observed in the eukaryote crown group. So far, they are relatively rare in the prokaryotes, and are restricted to the well-characterized sensory rhodopsins of various phototropic prokaryotes.

Results

Utilizing the currently available wealth of prokaryotic genomic sequences, we set up a computational screen to identify putative 7 (TM) and other multi-pass membrane receptors in prokaryotes. As a result of this procedure we were able to recover two widespread families of 7 TM receptors in bacteria that are distantly related to the eukaryotic 7 TM receptors and prokaryotic rhodopsins. Using sequence profile analysis, we were able to establish that the first members of t

SUBMITTER: Anantharaman V 

PROVIDER: S-EPMC212514 | biostudies-literature | 2003 Aug

REPOSITORIES: biostudies-literature