CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis.

ABSTRACT:

Rationale

Costimulatory molecules, including the CD40-CD154 and CD80/86-CD28 dyads, play a prominent role in regulating inflammation in the adaptive immune response. Studies from our group and others suggest a potentially important role for these costimulatory cascades in innate immunity as well.

Objectives

To determine the role of CD80/86 alone and in combination with CD40 in lethal polymicrobial sepsis in mice and humans.

Methods

The murine cecal ligation and puncture (CLP) model was used to determine the role of CD80/86 alone and in combination with CD40 using wild-type mice, CD80/86(-/-) mice, and novel CD40/80/86(-/-) mice. Expression of cell-bound and soluble costimulatory molecules was assessed in humans via ELISA and flow cytometry.

Measurements and main re

SUBMITTER: Nolan A 

PROVIDER: S-EPMC2218847 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature