Ontology highlight
ABSTRACT:
SUBMITTER: Moskovitz J
PROVIDER: S-EPMC23225 | biostudies-literature | 1997 Sep
REPOSITORIES: biostudies-literature
Moskovitz J J Berlett B S BS Poston J M JM Stadtman E R ER
Proceedings of the National Academy of Sciences of the United States of America 19970901 18
A gene homologous to methionine sulfoxide reductase (msrA) was identified as the predicted ORF (cosmid 9379) in chromosome V of Saccharomyces cerevisiae encoding a protein of 184 amino acids. The corresponding protein has been expressed in Escherichia coli and purified to homogeneity. The recombinant yeast MsrA possessed the same substrate specificity as the other known MsrA enzymes from mammalian and bacterial cells. Interruption of the yeast gene resulted in a null mutant, DeltamsrA::URA3 stra ...[more]