Optimal control and analysis of two-color genomotyping experiments using bacterial multistrain arrays.

ABSTRACT:

Background

Microarray comparative genomic hybridization (aCGH) evaluates the distribution of genes of sequenced bacterial strains among unsequenced strains of the same or related species. As genomic sequences from multiple strains of the same species become available, multistrain microarrays are designed, containing spots for every unique gene in all sequenced strains. To perform two-color aCGH experiments with multistrain microarrays, the choice of control sample can be the genomic DNA of one strain or a mixture of all the strains used in the array design. This important problem has no universally accepted solution.

Results

We performed a comparative study of the two control sample options with a Streptococcus pneumoniae microarray designed with three fully sequenced strain

SUBMITTER: Pinto FR 

PROVIDER: S-EPMC2410139 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature