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Anomalies in the influenza virus genome database: new biology or laboratory errors?

ABSTRACT: A search of the influenza virus genome database reveals anomalies associated with a nonnegligible number of submitted sequences. There are many pairs of viral segments that are very close to each other in nucleotide sequence but relatively far apart in reported time of isolation, resulting in an abnormally low evolutionary rate. Also, some sequences show clear evidence of apparent homologous recombination, a process normally assumed to be extremely rare or nonexistent in this virus. These findings may point to surprising new biology but are perhaps more readily explained by stock contamination or other errors in the sequencing laboratories.

SUBMITTER: Krasnitz M

PROVIDER: S-EPMC2519662 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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Anomalies in the influenza virus genome database: new biology or laboratory errors?

Krasnitz Michael M Levine Arnold J AJ Rabadan Raul R

Journal of virology 20080625 17

A search of the influenza virus genome database reveals anomalies associated with a nonnegligible number of submitted sequences. There are many pairs of viral segments that are very close to each other in nucleotide sequence but relatively far apart in reported time of isolation, resulting in an abnormally low evolutionary rate. Also, some sequences show clear evidence of apparent homologous recombination, a process normally assumed to be extremely rare or nonexistent in this virus. These findin ...[more]

PMID: 18579605

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