Haplotype-defined linkage region for gPRA in Schapendoes dogs.

ABSTRACT:

Purpose

In order to determine the molecular basis of canine generalized progressive retinal atrophy (gPRA), we initiated whole-genome scanning for linkage in gPRA-informative pedigrees of the Schapendoes breed.

Methods

Detailed pedigree and ophthalmological data were assembled in selected Schapendoes pedigrees. A whole-genome scan was initiated by two-point linkage analysis using microsatellite markers in combination with haplotype analyses. Mutation screening was carried out in respective candidate genes by DNA sequencing of amplified products and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).

Results

Genotyping data of the microsatellite genome scan evidenced a peak two-point lod score of 4.78 for marker REN93E07 on CFA20. Haplotype

SUBMITTER: Lippmann T 

PROVIDER: S-EPMC2533032 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature