Isolation of predation-deficient mutants of Bdellovibrio bacteriovorus by using transposon mutagenesis.

ABSTRACT: The availability of the complete genome sequence of Bdellovibrio bacteriovorus provides an opportunity for investigating genes that play a significant role in predation. Using two independently derived facultatively predatory Bdellovibrio strains, we have designed a method to cultivate and screen transposon insertion mutants in 96-well microtiter dishes. Transposon insertion mutants were produced by introducing the plasposon pRL27, which carries a mini-Tn5. Mutants have been screened for predatory activity using 96-well plates. Seventeen independent nonpredatory mutants have been isolated, and DNA flanking the insertion has been sequenced. BLAST analysis revealed that most of these interrupted DNA sequences do not code for known proteins or functions. Two of the inactivated genes were anal

SUBMITTER: Tudor JJ 

PROVIDER: S-EPMC2546638 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature