IFN-gamma impairs release of IL-8 by IL-1beta-stimulated A549 lung carcinoma cells.

ABSTRACT:

Background

Production of interferon (IFN)-gamma is key to efficient anti-tumor immunity. The present study was set out to investigate effects of IFNgamma on the release of the potent pro-angiogenic mediator IL-8 by human A549 lung carcinoma cells.

Methods

A549 cells were cultured and stimulated with interleukin (IL)-1beta alone or in combination with IFNgamma. IL-8 production by these cells was analyzed with enzyme linked immuno sorbent assay (ELISA). mRNA-expression was analyzed by real-time PCR and RNase protection assay (RPA), respectively. Expression of inhibitor-kappa Balpha, cellular IL-8, and cyclooxygenase-2 was analyzed by Western blot analysis.

Results

Here we demonstrate that IFNgamma efficiently reduced IL-8 secretion under the influence of IL-1beta. Surpr

SUBMITTER: Boost KA 

PROVIDER: S-EPMC2556346 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature