High-throughput Agrobacterium-mediated barley transformation.

ABSTRACT:

Background

Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop in the world. It also provides a useful model for the study of wheat, which has a larger and more complex genome. Most existing barley transformation methodologies are either complex or have low (<10%) transformation efficiencies.

Results

A robust, simple and reproducible barley transformation protocol has been developed that yields average transformation efficiencies of 25%. This protocol is based on the infection of immature barley embryos with Agrobacterium strain AGL1, carrying vectors from the pBract series that contain the hpt gene (conferring hygrom

SUBMITTER: Bartlett JG 

PROVIDER: S-EPMC2562381 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature