Identification of Mannheimia haemolytica adhesins involved in binding to bovine bronchial epithelial cells.

ABSTRACT: Mannheimia haemolytica, a commensal organism of the upper respiratory tract in cattle, is the principal bacterial pathogen associated with the bovine respiratory disease complex. Adherence to the respiratory mucosa is a crucial event in its pathogenesis. However, the bacterial components that contribute to this process are not fully characterized. In this study, we demonstrated that M. haemolytica adhered to bovine bronchial epithelial cells (BBEC) in vitro and that adherence was inhibited by anti-M. haemolytica antibody. Western blot analysis of M. haemolytica proteins that bind to BBEC showed a dominant protein band with an apparent molecular mass of approximately 30 kDa. Peptide sequences for the 30-kDa BBEC-binding proteins, as determined by liquid chromatography-tandem mass spectromet

SUBMITTER: Kisiela DI 

PROVIDER: S-EPMC2612293 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature