Analysis by mutagenesis of a chromosomal integron integrase from Shewanella amazonensis SB2BT.

ABSTRACT: Integrons are mobile genetic elements that can integrate and disseminate genes as cassettes by a site-specific recombination mechanism. Integrons contain an integrase gene (intI) that carries out recombination by interacting with two different target sites; the attI site in cis with the integrase and the palindromic attC site of a cassette. The plasmid-specified IntI1 excises a greater variety of cassettes (principally antibiotic resistance genes), and has greater activity, than chromosomal integrases. The aim of this study was to analyze the capacity of the chromosomal integron integrase SamIntIA of the environmental bacterium Shewanella amazonensis SB2BT to excise various cassettes and to compare the properties of the wild type with those of mutants that substitute consensus residues of

SUBMITTER: Larouche A 

PROVIDER: S-EPMC2648364 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature