Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock.

ABSTRACT: Shewanellae are microbial models for environmental stress response; however, the sequential expression of mechanisms in response to stress is poorly understood. Here we experimentally determine the response mechanisms of Shewanella amazonensis SB2B during sodium chloride stress using a novel liquid chromatography and accurate mass-time tag mass spectrometry time-course proteomics approach. The response of SB2B involves an orchestrated sequence of events comprising increased signal transduction associated with motility and restricted growth. Following a metabolic shift to branched chain amino acid degradation, motility and cellular replication proteins return to pre-perturbed levels. Although sodium chloride stress is associated with a change in the membrane fatty acid composition in other

SUBMITTER: Parnell JJ 

PROVIDER: S-EPMC3216512 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature