High resolution preparation of monocyte-derived macrophages (MDM) protein fractions for clinical proteomics.

ABSTRACT:

Background

Macrophages are involved in a number of key physiological processes and complex responses such as inflammatory, immunological, infectious diseases and iron homeostasis. These cells are specialised for iron storage and recycling from senescent erythrocytes so they play a central role in the fine tuning of iron balancing and distribution. The comprehension of the many physiological responses of macrophages implies the study of the related molecular events. To this regard, proteomic analysis, is one of the most powerful tools for the elucidation of the molecular mechanisms, in terms of changes in protein expression levels.

Results

Our aim was to optimize a protocol for protein fractionation and high resolution mapping using human macrophages for clinical studies. We

SUBMITTER: Polati R 

PROVIDER: S-EPMC2649903 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature