Transient and etiology-related transcription regulation in cirrhosis prior to hepatocellular carcinoma occurrence.

ABSTRACT:

Aim

To search for transcription dysregulation that could (1) differentiate hepatocellular carcinoma (HCC)-free from HCC-related cirrhosis (2) differentiate HCC-free cirrhosis related to HCV from that related to alcohol intake.

Methods

Using microarray analysis, we compared transcript levels in HCC-free cirrhosis (alcoholism: 7; hepatitis C: 7), HCC-associated cirrhosis (alcoholism: 10; hepatitis C: 10) and eight control livers. The identified transcripts were validated by qRT-PCR in an independent cohort of 45 samples (20 HCC-free cirrhosis; 15 HCC-associated cirrhosis and 10 control livers). We also confirmed our results by immunohistochemistry.

Results

In HCC-free livers, we identified 70 transcripts which differentiated between alcoholic-related cirrhosis, HCV-rela

SUBMITTER: Caillot F 

PROVIDER: S-EPMC2653326 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature