Quantitative monitoring of insulin secretion from single islets of Langerhans in parallel on a microfluidic chip.

ABSTRACT: Quantification of insulin release from pancreatic islets of Langerhans is of interest for diabetes research. Typical insulin secretion experiments are performed using offline techniques that are expensive, slow, have low-throughput, and require multiple islets. We have developed a microfluidic device for high-throughput, automated, and online monitoring of insulin secretion from individual islets in parallel. This chip consists of 15 channel networks each capable of superfusing a single islet and mixing superfusate from each islet online with fluorescein isothiocyanate-labeled insulin and anti-insulin antibody for a competitive immunoassay. The resulting continuous reaction streams are periodically injected onto parallel electrophoresis channels where the mixtures are separated. The result

SUBMITTER: Dishinger JF 

PROVIDER: S-EPMC2679996 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature