Evidence that human blastomere cleavage is under unique cell cycle control.

ABSTRACT:

Purpose

To understand the molecular pathways that control early human embryo development.

Methods

Improved methods of linear amplification of mRNAs and whole human genome microarray analyses were utilized to characterize gene expression in normal appearing 8-Cell human embryos, in comparison with published microarrays of human fibroblasts and pluripotent stem cells.

Results

Many genes involved in circadian rhythm and cell division were over-expressed in the 8-Cells. The cell cycle checkpoints, RB and WEE1, were silent on the 8-Cell arrays, whereas the recently described tumor suppressor, UHRF2, was up-regulated >10-fold, and the proto-oncogene, MYC, and the core element of circadian rhythm, CLOCK, were elevated up to >50-fold on the 8-Cell arrays.

Conclusions

T

SUBMITTER: Kiessling AA 

PROVIDER: S-EPMC2682187 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature