Automated high-dimensional flow cytometric data analysis.
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ABSTRACT:
SUBMITTER: Pyne S
PROVIDER: S-EPMC2682540 | biostudies-literature | 2009 May
REPOSITORIES: biostudies-literature
Publications
Automated high-dimensional flow cytometric data analysis.
Pyne Saumyadipta S Hu Xinli X Wang Kui K Rossin Elizabeth E Lin Tsung-I TI Maier Lisa M LM Baecher-Allan Clare C McLachlan Geoffrey J GJ Tamayo Pablo P Hafler David A DA De Jager Philip L PL Mesirov Jill P JP
Proceedings of the National Academy of Sciences of the United States of America 20090514 21
Flow cytometric analysis allows rapid single cell interrogation of surface and intracellular determinants by measuring fluorescence intensity of fluorophore-conjugated reagents. The availability of new platforms, allowing detection of increasing numbers of cell surface markers, has challenged the traditional technique of identifying cell populations by manual gating and resulted in a growing need for the development of automated, high-dimensional analytical methods. We present a direct multivari ...[more]
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