Identification of microbial DNA in human cancer.

ABSTRACT:

Background

Microorganisms have been associated with many types of human diseases; however, a significant number of clinically important microbial pathogens remain to be discovered.

Methods

We have developed a genome-wide approach, called Digital Karyotyping Microbe Identification (DK-MICROBE), to identify genomic DNA of bacteria and viruses in human disease tissues. This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library.

Results

To validate this technology and to identify pathogens that may be associated with human cancer pathogenesis, we used DK-MICROBE to determine the presence of micr

SUBMITTER: Duncan CG 

PROVIDER: S-EPMC2685141 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature