Ontology highlight
ABSTRACT:
SUBMITTER: Butter F
PROVIDER: S-EPMC2697111 | biostudies-literature | 2009 Jun
REPOSITORIES: biostudies-literature
Butter Falk F Scheibe Marion M Mörl Mario M Mann Matthias M
Proceedings of the National Academy of Sciences of the United States of America 20090616 26
Mass spectrometry (MS)-based quantitative interaction proteomics has successfully elucidated specific protein-protein, DNA-protein, and small molecule-protein interactions. Here, we developed a gel-free, sensitive, and scalable technology that addresses the important area of RNA-protein interactions. Using aptamer-tagged RNA as bait, we captured RNA-interacting proteins from stable isotope labeling by amino acids in cell culture (SILAC)-labeled mammalian cell extracts and analyzed them by high-r ...[more]